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Automated Systems

The Interchim range of automated flash chromatography equipment (puriFlash), provides a comprehensive solution to the varied requirements of the chemistry, medicinal chemistry and multifunctional laboratory.

puriFlash<sup>®</sup> 210
Robust entry level budget flash chromatography system with comparable features to traditional Flash systems.
puriFlash<sup>®</sup> 430
Quaternary gradient, 30 bar pressure capability within a modular chassis for increased ease of use. Delivers enhanced flash chromatography and entry level preparative capability through Interchim’s patented dual piston pump.
puriFlash<sup>®</sup> 450
Interchim’s flagship Flash Chromatography / Flash Purification system, with quaternary pump and 50 bar pressure capability. Integrated injection valve for liquid or solid injection facilitating 1.6KG flash cartridges and 3” LC prep columns.
puriFlash<sup>®</sup> 4100
A true Flash Chromatography to Preparative system for the multifunctional laboratory. High through-put work station with 100 bar pressure capability using Interchim’s patented dual piston quaternary pump for solvent delivery.
puriFlash<sup>®</sup> 800
800 ml per minute flow rates achieved through modifications to the binary pump and solvent mixing chamber.

Features

PF-210 PF-430 PF-450 PF-4100 PF-800
Pump
Flow rate 150ml / min 200ml / min 250ml / min 250ml / min 800ml / min
Pressure max (bar) 10 30 50 100 10
Gradient Binary Quaternary Quaternary Quaternary Binary
Air purge Option Option Yes Yes No
Washing disc No Yes Yes Yes Yes
Detection
UV (nm) 254 (default) 200-600 200-600 200-600 200-600
Flow cell optical length (mm) 0.3 0.3 0.3 0.3 0.3
Second detector channel Yes Yes Yes Yes Yes
Injection
Direct (fixed holder) Option Yes Yes Yes Yes
6 way electrical valve Option Option Yes Yes Option
Injection mode: Liquid - Dry load Yes Yes Yes Yes Yes
Large injection volume by external pump Yes Yes Yes Yes Yes
Column Holder
Integrated Option Yes Yes Yes Yes
Stand alone w/ 4-way valve Option Option No No No
Pre-column holder Option Option Yes Yes Option
Extension kit for large columns Option Option Yes Yes Yes
Unit Control
Touch Screen (10.5") Yes Yes Yes Yes Yes
USB 2 4 4 4 4
RJ45/ RJ11 Yes Yes Yes Yes Yes
Flexible keyboard Option Yes Yes Yes Yes
Software
puriFlash software (ver 5.0) Yes Yes Yes Yes Yes
Fraction collector
Racks 4 racks - short 4 racks - short 4 racks - short 4 racks - short 4 racks - short
Solvent Tray
Tray Option Yes Yes Yes Yes

Automated Flash purification

Automated Flash purification came to the fore a generation ago with the Jones FlashmasterTM being one of the first systems to allow automated chemical cleanup using silica beads in glass columns and latterly, cartridges. The next generation of automated systems, such as the Biotage® SP4TM, Biotage® IsoleraTM and ISCO® companion® established enhanced ease of use with improvements in accuracy and system pressure capability to manage developments in cartridge features.

Interchim released their first puriFlash® Flash Chromatography system in 2008. They recognised enhanced purification possibilities with current cartridge / silica (media) developments and a need to support the multifunctional requirements within the modern laboratory. The Interchim Flash Chromatography range (excluding PF-210) combines innovation in pump technology with innovation in cartridge production and silica capability. This combination facilitates reduced purification steps, cleaner fractions, up to 3-fold reduction on run times and 4-fold increases in sample loading over current mainstream flash. We call this Ultra Pressure Flash Purification (UPFP).

The Preparative twist

Capital equipment that can serve a diverse range of projected and unforeseen applications provides organisations with extensive cost savings for potential multiple system purchases and flexibility within their purification demands.

The higher pressure Interchim puriFlash range has been utilised worldwide for diverse and problematic purification applications that competitor flash systems are unable to address.

These allow for a seamless switching from a standard flash cartridge through to reduced micron, high pressure high speed flash cartridges and ultimately to stainless steel semi-preparative and preparative HPLC columns. The quaternary gradient pump along with multi-detector options support an extensive range of preparative requirements.

This capability creates a system that serves a laboratory’s or department’s multifunctional demand – from routine, day –to- day clean-up procedures to more complex purifications as experienced by a number of our clients with crude mixtures or enantiomers.

3-fold reduction in run times

Graph 1 Graph 2 Graph 3 Graph 4
Puriflash Puriflash Puriflash Puriflash
50µm PuriflashTMSilica Std, 25g
#: IR-50SI/25G
Flow Rate: 20ml/ min
Back Pressure: 0 Bar
Solvents: A: Hexane, B: Ethyl Acetate
Grad CV: 0.0 - 9.6 CV, 20% B
Grad Time: 0.00 - 24.00min, 20% B
Sample: 2ml, 500mg/ml, 4% load
50µm PuriflashTMSilica HP, 25g
#: PF-50SIHP/25G
Flow Rate: 20ml/ min
Back Pressure: 0 Bar
Solvents: A: Hexane, B: Ethyl Acetate
Grad CV: 0.0 - 4.8 CV, 0-20% B, 4.8- 5.6 CV, 20% B
Grad Time: 0.0 - 12.0, 0-20% B, 12.0 - 15.0, 20% B
Sample: 2ml, 500mg/ml, 4% load
30µm PuriflashTMSilica HP, 25g
#: PF-30SIHP/25G
Flow Rate: 30ml/ min
Back Pressure: 1 Bar
Solvents: A: Hexane, B: Ethyl Acetate
Grad CV: 0.0 - 4.8 CV, 0-20% B, 4.8- 5.6 CV, 20% B
Grad Time: 0.0 - 8.0, 0-20% B, 8.0 - 10.0, 20% B
Sample: 2ml, 500mg/ml, 4% load
15µm PuriflashTMSilica HP, 25g
#: PF-15SIHP/25G
Flow Rate: 40ml/ min
Back Pressure: 2 Bar
Solvents: A: Hexane, B: Ethyl Acetate
Grad CV: 0.0 - 4.8 CV, 0-20% B, 4.8- 5.6 CV, 20% B
Grad Time: 0.0 - 6.0, 0-20% B, 6.0 - 7.0, 20% B
Sample: 2ml, 500mg/ml, 4% load

Increased resolution combined with run time reduction

Puriflash

4-fold increase in sample load

Graph 1 Graph 2 Graph 3
Increase in sample load Increase in sample load Increase in sample load
50µm PuriflashTMSilica HC, 25g
#: PF-50SIHC/25G
Flow Rate: 20ml/ min
Back Pressure: 0 Bar
Solvents: A: Hexane, B: Ethyl Acetate
Grad CV: 0.0 - 4.8 CV, 0 - 20% B, 4.8 - 6.4 CV, 20% B
Grad Time: 0.0 - 12.0min, 0 - 20% B, 12.0 - 16.0, 20% B
Sample: 5ml, 500mg/ ml, 2.5g, 10% load
50µm PuriflashTMSilica HC, 25g
#: PF-50SIHC/25G
Flow Rate: 20ml/ min
Back Pressure: 0 Bar
Solvents: A: Hexane, B: Ethyl Acetate
Grad CV: 0.0 - 4.8 CV, 0 - 20% B, 4.8 - 6.4 CV, 20% B
Grad Time: 0.0 - 12.0min, 0 - 20% B, 12.0 - 16.0, 20% B
Sample: 7ml, 500mg/ ml, 3.5g, 14% load
50µm PuriflashTMSilica HC, 25g
#: PF-50SIHC/25G
Flow Rate: 20ml/ min
Back Pressure: 0 Bar
Solvents: A: Hexane, B: Ethyl Acetate
Grad CV: 0.0 - 4.8 CV, 0 - 20% B, 4.8 - 6.4 CV, 20% B
Grad Time: 0.0 - 12.0min, 0 - 20% B, 12.0 - 16.0, 20% B
Sample: 10ml, 500mg/ ml, 5.0g, 20% load

Flash to preparative transposition

Puriflash

Flash to preparative transposition (Univ Munich)
30 micron 40 Gram flash transposed to 10 micron prep LC (250 x 10mm)

puriFlash Pump

Puriflash pump

Interchim's patented quaternary pump is at the heart of the purification process in the higher pressure systems. puriFlash® 430, 450 and 4100 systems deliver unparalleled accuracy in the flash environment. The dual piston pump offers true low volume flow rates to 1ml / min within an accuracy of +/- 1% and up to 100 bar pressures at 250ml/ min flow rates in the 4100 system.

The quaternary design allows you to simultaneously mix 4 channels at 1% accuracy (competitor systems quaternary options generally allow mixing of 2 channels at a time) and resolves headaches associated with pre-mixing and running shallow gradients.

Puriflash

puriFlash Software

puriFlash® version 5 software provides the chemist with the easiest interface yet for purification management. Users can create a method and control the purification process up to report generation from a single screen.

Puriflash Software

puriFlash Fraction Collector

Puriflash Software

The puriFlash® fraction collector benefits from 4 front-loading racks that maximise sample volume collection, flexibility and ease of use.

Tube Size mm No. of tubes Tube Vol. ml Total Vol. ml
13 x 100 240 9 2160
16 x 150 176 22 3872
18 x 150 176 25 4400
18 x 180 176 32 5632
21 x 150 108 35 3780
25 x 150 64 60 3840
28 x 150 56 75 4200
29.5 x 200 56 110 6160
250ml 3 x 2 250 1500
500ml 3 x 2 500 3000

UV Detection

UV Detection

UV detection is supplied as standard with all Interchim systems. The entry level 210 system is supplied with a fixed wavelength (254nm std). The rest of the range is supplied with an adjustable 200 - 600nm wavelength with dual wavelength collection. This can be increased to 840nm as an option. Flow cell optimal length is 0.3mm.

Integrating your puriFlash system with ELS detection (ELSD)

Cartridge Holder

Cartridge holder

The puriFlash® range carries an integrated cartridge holder which holds flash cartridges from 4 -to- 330 gram sizes (optional on a 210 system). puriFlash® 450 and 4100 also feature an extension kit for larger cartridges a pre-column holder for a dry or pre-load cartridges and the Interchim Load & GoTMautomated loop injection valve that manages liquid and solid injection with column equilibration.

 

 

System evaluation, application data and consideration

Cheshire Sciences have vastly experienced technical representatives to provide intuitive, tailored demonstrations relative to your organisational set-up in the United Kingdom.

Equipment Leasing

We offer a number of attractive equipment leasing options to spread equipment costs over 1 to 5 years with excellent tax efficiency.

Global Network

Interchim have a global network of subsidiaries and distributors to service all requirements across the globe

Demonstration?

For more information and a potential demonstration complete the enquiry form at the top of the navigation bar on the left of the page.

Pricing Information

Systems, Parts & Accessories