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SDS-Gel Stains

SDS-Gel Stains

CooBlue MAX & CooBlue FX - Coomassie based detection

  • 10 - 20ng sensitivity for most proteins
  • Ready -to- Use
  • Low cost

'Home Made' Coomassie formulations have been popular for protein visualisation in SDS PAGE electrophoresis gels for over a generation.

Interchim have developed ready-to-use instant staining Uptima products based upon a Coomassie blue colloidal formulations.

CooBlue MAX

CooBlue MAX are for those researchers who demand optimal sensitivity and clarity from their Coomassie based protein detection in SDS PAGE Gel electrophoresis. Selective Protein bands are visible below 10ng and most proteins to 20ng levels. CooBlue MAX contains minimal quantities of organic solvents to maximise sensitivity and clarity as per the popular brands supplied on the market.

CooBlue FX

Displaying nearly all of the sensitivity of CooBlue MAX under the same simple protocols but containing no trace of organic solvents. CooBlue FX is our environmentally friendly formula with selective Protein bands visible down to 10ng (protein dependant) . Bands are readily seen upon immediate staining and a simple wash step enhances the band clarity.

Product No. Component Volume Description Instructions MSDS Price
R2034e 30ml CooBlue MAX instant protein detection in SDS PAGE Gels.. pdf pdf £12.00
UPR2034A 500ml CooBlue MAX instant protein detection in SDS PAGE Gels.. pdf pdf £29.90
UPR2034B 4.5 litres CooBlue MAX instant protein detection in SDS PAGE Gels.. pdf pdf £195.00
G4562e 30ml CooBlue FX instant protein detection in SDS PAGE Gels.. pdf pdf £12.00
UPG4562A 500ml CooBlue FX instant protein detection in SDS PAGE Gels.. pdf pdf £29.90
UPG4562B 4.5 litres CooBlue FX instant protein detection in SDS PAGE Gels.. pdf pdf £195.00
T34711 1 Accessory pump for 4.5 litre Cooblue.. £12.00
Test Data

Cool Blue Test Data

General Considerations

CoolBlue FX / MAX

Overview

  • Ready-to-use reagent
  • Sensitivity <10ng (protein dependent)
  • MS compatible
  • 12 months stability
  • No methanol/ acetic acid required
  • Microwave protocol option

* Important! Always mix the bottle of stain thoroughly before use to give a homogenous solution - SDS interferes with the assay, wash extensively to make sure that all SDS is removed from the gel before staining

Whats the difference between CooBlue FX and CooBlue MAX

CooBlue FX is a ‘safer’ option in terms of : environmentally, health wise and for shipping as it contains no trace organic solvents. FX is a little lighter in colour compared to CooBlue Max and slightly less sensitive.

After using CooBlue is MS and sequencing possible?

Yes. Although de-staining is never 100%. We therefore would recommend CooBlue Stain # UP47255A or ProSave Reversible Stain # BP7121

My stain has stopped working / is not working well. What can I do?

The stain needs to be mixed thoroughly before each use to make sure that you get a homogenous solution. Mix well and try again.