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Calcium Indicators

Fluorescent

Fluo-8

  • 2x Fluo-4 brightness, 4x Fluo-3 brightness
  • Absorption at 490nm / Emission at 514nm
  • Room temperature loading without loss of signal
  • Enhanced photostability
  • Price competitive

Fluo-8 is the latest reagent for Ca2+ detection. It is twice as bright as Fluo-4 and 4x brighter than Fluo-3 with absorption peaks at 490nm and emission peaks at 514nm.

Fluo-8 is more readily loaded than its predecessors, either at room temperature or 37°C it also displays enhanced photostability making it useful to detect transient Ca2+ levels.

Fluo-8 is available in a number of pack sizes as AM esters for live cell membrance access, sodium salts and formulations for high and low concentrations of calcium.

Product No. Description Qty. Kd (Calcium) MW (g-1, mol-1) Technical Sheet MSDS Price
CP7501 Fluo-8 AM 5x 50g 389nM 1000 PDF PDF £93.40
CP7502 Fluo-8 AM. 10x 50 g 389nM 1000 PDF PDF £171.80
CP7503 Fluo-8 AM. 20x 50 g 389nM 1000 PDF PDF £289.80
CP7504 Fluo-8 AM. 1 mg 389nM 1000 PDF PDF £240.70
CP7520 Fluo-8, Sodium Salt 10x 50 g 389nM 1000 PDF PDF £142.50
CP7531 Fluo-8H, AM (for high calcium concentrations) 10x 50 g 232nM 1100 £191.60
CP7530 Fluo-8H, AM (for high calcium concentrations) 1 mg 232nM 1100 £289.80
CP7540 Fluo-8H, Sodium Salt (for high calcium concentrations) 10x 50 g 232nM 1100 £289.80
CP7551 Fluo-8L, AM (for low calcium concentrations) 10x 50 g 1.86µm 1100 £191.60
CP7550 Fluo-8L, AM (for low calcium concentrations) 1 mg 1.86µm 1100 £289.80
CP7560 Fluo-8L, Sodium Salt (for low calcium concentrations) 10x 50 g 1.86µm 1100 £289.80

Luminescent / Substrates

Coelenterazine

Coelenterazine and their derivatives display unique luminescent properties providing extensively referenced benefits for cell studies. They display several advantages compared to fluorescent indicators. Our products are ultra pure and are available at excellent prices compared to leading brands.

  • Complexes can detect a broad range of calcium concentrations
  • Lower backgrounds with no autofluorescence concerns
  • Higher signal-to-noise ratios can be obtained with imaging equipment processes
  • Changes in cell Calcium concentration can be monitored for long times as the aequorin complex is retained
  • Excellent prices
  • Ultra pure

Further information regarding each coelenterazine derivatives

Product No. Description MW Qty. Price
UP972331 Coelenterazine, Native 423.50 50 g £58.00
UP972333 Coelenterazine, Native 423.50 1 mg £133.30
UPR30791 Coelenterazine, cp 415.48 50 g £58.00
UPR30793 Coelenterazine, cp 415.48 1 mg £201.10
UP438761 Coelenterazine, f 425.45 50 g £58.00
UPR38763 Coelenterazine, f 425.45 1 mg £201.10
UPR47110 Coelenterazine, fcp 417.48 50 g £58.00
UPR47111 Coelenterazine, fcp 417.48 1 mg £533.00
UPR30782 Coelenterazine, h 407.50 50 g £58.00
UPR30783 Coelenterazine, h 407.50 1 mg £133.30
UP083532 Coelenterazine, hcp 399.49 50 g £58.00
UP083534 Coelenterazine, hcp 399.49 1 mg £201.10
UPR30801 Coelenterazine, i 533.36 50 g £58.00
UPR30803 Coelenterazine, i 533.36 1 mg £533.00
UPR47120 Coelenterazine, ip 389.45 50 g £58.00
UPR47122 Coelenterazine, ip 389.45 1 mg £533.00
UP398192 Coelenterazine, n 457.52 50 g £58.00
UP398193 Coelenterazine, n 457.52 1 mg £201.10
FP42176C Coelenterazine Sampler Kit 25g ea £146.30
UPT88890 Coelenterazine, 2-methyl 331.37 50 g £43.00
UPT88891 Coelenterazine, 2-methyl 331.37 1 mg £134.00
UPBB8391 Coelenterazine, 400a 391.46 50 g £61.70
UPBB8392 Coelenterazine, 400a 391.46 1 mg £134.00

D-Luciferin

D-Luciferin is a luminescent substrate for firefly luciferase. The luciferin / luciferase system is primarily used for reporter and ATP assays. Our products are ultra pure and are less expensive than leading brands.

  • High purity <99% elimates problems associated with luciferase inhibitors
  • Excellent prices
  • K salts available from stock

Luminescent Calcium Instructions

Product No. Description MW Qty. Price
FP-27060A D-luciferin, Free Acid 280.33 10 mg £93.40
FP-27060B D-luciferin, Free Acid 280.33 250 mg £171.80
FP-27060D D-luciferin, Free Acid 280.33 1 gram £568.20
FP-72604A D-luciferin, Na Salt 302.30 25 mg £83.50
FP-72604B D-luciferin, Na Salt 302.30 50 mg £146.30
FP-72604C D-luciferin, Na Salt 302.30 1 gram £568.20
FP-M1224A D-luciferin, K Salt 318.42 25 mg £97.40
FP-M1224B D-luciferin, K Salt 318.42 50 mg £137.00
FP-M1224C D-luciferin, K Salt 318.42 500 mg £415.20
FP-M1224D D-luciferin, K Salt 318.42 1 gram £471.00
FP-CF4420 D-luciferin, ethyl ester 306.36 2 mg £86.80
FP-CF4421 D-luciferin, ethyl ester 306.36 10 mg £303.90
FP-M1418A D-luciferin, 6 methyl ester 294.30 10 mg £97.40